In vitro toxicology has progressed hugely over the past decades and is now seen as a science in itself. In line with the 3Rs concept, ERBC performs ICH and OECD compliant tests to identify toxicology events in nonwhole animal models (cell cultures, 3D models…).
Rapid, reproducible and cost-effective, these assays contribute to enhance R&D productivity and minimize the use of animals.
Skin corrosion
- Membrane barrier test method for skin corrosion (Corrositex®)
- Human skin model test (Episkin™)
Skin irritation
- Human skin model test (Episkin™)
Acute oral systemic toxicity
- Balb/C 3T3 neutral red uptake
Phototoxicity
- Balb/C 3T3 cell phototoxicity assay (neutral red uptake)
Cytotoxicity
- Test by direct or indirect contact in clone 929, Balb/C 3T3, V79
In vitro wound healing
- Cell proliferation and migration in primary normal human keratinocytes
Hepatotoxicity
- Cytotoxicity in primary rat hepatocytes
- HepG2 cell screening test
Nephrotoxicity
- LLC-RK1 cell screening test
Hæmatotoxicity
- Colony forming unit granulocyte/macrophage assay
- Red blood cell haemolysis test
In vitro cancer screen
Endpoints
- Mitochondrial Reductase Activity (MTT Staining)
- Sulforhodamine B Uptake
Cell line panels
- Non-Small Cell Lung Cancer (NSCLC): A549, NCI-H23, NCI-H460
- Colon cancer: HCT-116, HT29, SW-620
- Ovarian cancer: OVCAR-3, SK-OV-3
- Prostate cancer: PC-3, DU-145
- Breast tumor: MCF7, MDA-MB-231, HS 578T
