In vitro toxicology has progressed hugely over the past decades and is now seen as a science in itself. In line with the 3Rs concept, ERBC performs ICH and OECD compliant tests to identify toxicology events in nonwhole animal models (cell cultures, 3D models…).
Rapid, reproducible and cost-effective, these assays contribute to enhance R&D productivity and minimize the use of animals.
- Membrane barrier test method for skin corrosion (Corrositex®)
- Human skin model test (Episkin™)
- Human skin model test (Episkin™)
- Balb/C 3T3 neutral red uptake
- Balb/C 3T3 cell phototoxicity assay (neutral red uptake)
- Test by direct or indirect contact in clone 929, Balb/C 3T3, V79
- Cell proliferation and migration in primary normal human keratinocytes
- Cytotoxicity in primary rat hepatocytes
- HepG2 cell screening test
- LLC-RK1 cell screening test
- Colony forming unit granulocyte/macrophage assay
- Red blood cell haemolysis test
Endpoints
- Mitochondrial Reductase Activity (MTT Staining)
- Sulforhodamine B Uptake
Cell line panels
- Non-Small Cell Lung Cancer (NSCLC): A549, NCI-H23, NCI-H460
- Colon cancer: HCT-116, HT29, SW-620
- Ovarian cancer: OVCAR-3, SK-OV-3
- Prostate cancer: PC-3, DU-145
- Breast tumor: MCF7, MDA-MB-231, HS 578T
Mouse
Rat
Dog
Rabbit
Marmoset
Ferret
Cynomolgus Monkeys
Guinea Pig
